Blotting: a basic molecular biology technique originally created by
for locating gene specific sequences on DNA fragments isolated from cell.
BLOTTING*: is used to locate and identify genes on DNA fragments.
Restriction Fragments are electrophoretically separated and then melted to give ssDNA pieces.
The fragments are blotted onto nitrocellulose or nylon membranes, where the DNA bonds
permanently. Hybridization with labeled DNA probes & autoradiography localize target DNAs.
To find a gene one must have a DNA sequence that is complimentary to some region on fragments.
NORTHERN BLOTTING: a
variation on Southern blotting (a humorous analogy?) where a cell's
RNA's are separated by electrophoresis, transferred to filter paper, and probed with a labeled
another variation involves separating proteins via electrophoresis and then
probing the resulting filter paper with an antibody that binds to the protein your are interested