|click on a topic for more info|
The draft of the Human Genome
is only the beginning.
University of Miami Home Page |
Biology Department Home Page |
Dr. Mallery's Home Page
Human Genome Project... "The human genome is a parts list." Eric Lander October 2001
|The Human Genome Project||
of Energy HGP Site
National Institutes of Health HGP site
UK's Human Genome web site
Center for Human Genome Studies
DOE "Primer" on Genomics
| Whose genes
are being sequenced?
Criteria for whose gene are to be sequenced.
Celera Genomics sequenced the genomes of five
anonymous individuals - one African-Amerian, one Asian-
Chinese, one Hispanic-Mexican and two Caucasians. One
individual's genome was sequenced 3.5 times; about half
the genome of each of the remaining individuals was sequenced.
|The Sequencing Tools||
Experimental Techniques &
Restriction enzymes glossary
|Single Nucleotide Polymorphisms|
Cambridge Institute Long list of Glossaries
HGP Genome Glossary
Genomics & Proteomic Glossary
Biotech Media Resource Site
Dictionary of Genetic Terms
HHMI Glossary of Terms
National Plant Genome Glossary
European Bioinformatics Institute
|Genomics Industry||Gene Companies top|
Proteomics...is determining 3D protein
integrated relationships of all of a cell's proteins.
Often referred to as structural genomics, this new scientific discipline involves determining 3D structures of
large numbers of proteins. The goal of structural proteomics is to reveal the structures of all the key
"functional" sites of any human protein, which should make it much easier to develop highly specific drugs.
|Proteomics & the Proteomics Industry||Proteomics Companies|
|What is Structural Proteomics||How to determine structure|
|Known Protein Structures (3K to date)||Protein Data Bank|
|Protein Structure Prediction||PROSPECT|
|Protein Families||Protein Structure Initiative|
Human Proteomics Initiative
| Individual Species
Genomic Modeling Projects
Eukaryote model organisms
|Proteomics Glossary of Terms||CHI Glossary|
|Human Chromosome Maps||
Science: A gene map of the Human chromosomes
Gene Map '99
ORNL - Human Chromosome Maps
- Human Chromosome Launchpad
European Bioinformatics Institute
|Genealogy Maps||deCode & Iceland - Hunt for Disease Genes|
|Human Genetic Diseases||
Blazing a Gentic Trail - HHMI
Catalog of Human Diseases & Genetic Disorders
are self–renewing embryonic cells
that will develop into functional, differentiated cells. Human embryonic stem
cells are pluripotent, that is, they can
all cells and tissues in the body. Two types of human embryonic stem cells:
1. human embryonic stem (hES) cells, derived by researchers from donated in vitro fertilized
blastocysts or early-stage embryos,
2. and human embryonic germ (hEG) cells, which were derived from donated fetal material.
|Mallery's Stem Cell page||A description of Stem Cells|
|Primers on Stem Cells||
Stem Cell Basics
NIH Stem Cell Information Site
|Stem Cell Labs||NIH Stem Cell Registry|
|Popular Press Reports||
Time.com - The Stem Cell Debate
CNN.com - The Stem Cell Debate
|Pictures of Stem Cells||Pictures|
|Personal Stem Cell Preservation||
4. New Born Blood Banking 5. LifeBank
|Stem Cell Companies||StemCells & Diseases of CNS|
Genomics - the ethical, legal, and social issues of gene research
The HGP Project will eventually allow geneticists to screen, diagnose, prevent
and/or treat many genetic disease states.
Would you want to test yourself for a genetic condition that had no cure?
Is your genetic data private? (Insurance companies, employers, potential spouse)
How will society (the courts) treat "undesirable" human behavioral traits?
What effect will "false positives" have on our "psyche"?
|Exploring Genetic Issues||Your Genes, Your Choices|
|American Journal of Bioethics||Bioethics.net|
|National Bioethics Advisory Commission||NBAC|
|U Pennsylvania||Center for Bioethics- uPenn|
|Human Genome Project||A primer on Societal Concerns|
How is the
DNA of the Genome studied?
Journal of DNA
The Tools of the Genetic Engineering - sophisticated & elegant lab techniques in Molecular Biology.
List of Molecular Biology Protocols
Isolation of DNA from tissues of interest (or
anyone can do it?
for HGP mostly from human white blood cells. (or from onion cells).
2. cut up isolated chromosome DNA via restriction endonucleases. (Arber Review)
types of restriction enzyme & where they cut
EcoRI - restriction endonuclease #1 from Escherichia coli.
3. make multiple copies of DNA fragments via gene cloning
DNA is spliced into vectors - plasmids, cosmids (Drosophilia cosmid Library)
- bacterial artificial chromosomes (BACs)
recombined DNA is copied (or cloned)
4. cloned pieces of DNA can undergo DNA sequencing... FAQ's
deoxy-nucleotides tagged with different colored fluorescent dyes...
Nature ref and commercial tags,
some of the protocols & methodologies
DNA copied then sequence read from fluorescent pattern when DNA lit with laser
commercial sequencing companies: SEQWright, CyberGene, Davis,
via fast-paced computer-run sequencing machine ABI-377 & ABI-6700
5. Sequencing strategies: shotgun & physical map constructs
chromosomal DNA cut by several restriction enzymes, cloned & sequenced
results in overlapping sequences which are aligned via computer analysis into physical map
top 6. Sequencing Yourself - NOVA Online's Human Genome Project site.