Electron Microscopy BIL 554
Techniques in transmission electron microscopy including tissue
preparation, use of the electron microscope, photography, and interpretation
Office: Cox 20
Meeting Time and Place:Tuesday, Thursday 1:40 to 5:15; Cox 20B.
In Depth Course Description:
Students will be trained to 1) start with a living organism or tissue, 2) fix (so cells appear in
suspended animaton), 3) imbed in a hard resin (permits thin section), 4) section
(with an ultramicrotome using glass knives), 5) examine the resulting thin
sections (40nm thick) using the Philips 300 transmission electron
microscope, 5) take pictures, 6) develope, 7) enlarage the resulting
negatives, 8) print them (using the EM darkroom), and finally 9) labelthe organelles and cell
structures visualized on the micrograph. Students are given individualized
instruction on all the above aspects involved with electron microscopy,
they master these techniques by practicing them either individualy or in a group, but the
final product is generated by their own, individual effort and work.
Operaton of the electron microscope, the ultramicrotomes and use of the darkrooms
ultimately is done by each student. This course will help the student
decide whether he/she would like to continue on in biological research
since the student must set their own schedules for mastering the
information (24 hr access to the laboratory and supporting facilities is
provided), must accomplish much of this on their own or in concert with
other members of the course, and must produce an accceptable product at several set times during
the course. Over .. of the students who take this course
continue on into professional schools; medical school or graduate school.
See BIL 555 for a list of student
projects in Electron Microscopy and the
professional programs these students entered since graduation from the
University of Miami--or CLICK HERE.
Ink gland of Aplysia californica, granulate cell
showing elaboration of the plasma membrane into coated vesicles
Admission to the course is only through permission of
the instructor. This involves filling out an application and having an
interview with the instructor during the spring semester (generally in
March) for the next fall semester's course offering. Eight
students are selected from the applicant pool and three to four others are chosen
as backups in case one of the eight is unable to schedule the course.
|Aug 29||1 R ||Introduction
|Sept 2||2 T ||Scope on; Large traps demo
| ||2 R||Check large traps; Knife making & thick
section demo; Eye lash stick
|Sept 10||3 T||Check thick section; Review large
trap & thick sect; Class scope on & do; Thin sect. demo.
| ||3 R||Class put on scope;Check small
traps; Thin sect. demo.
|Sept.17||4 T||Thin section review; Object. lense
| ||4 R ||Check thin sect. on grids; Staining
demo; Scope in groups of 3
|Sept 25||5 T||Review scope with 3's; Camera and film
load & develop; Thick sect. sketch
| ||5 R||Review camera and scope; Check camera load
|Oct 1||6 T||TEST
|Oct 8||7||INTERVIEWS and required tissues
|Oct 15||8||Jobs; Required tissues
|Oct 22||9||Required tissues & open lab
|Oct 29||10||Dark room; Low mag(Tap2)
|Nov 5||11||Low mag. prints
|Nov 12||12||High(Tap 12) and low mag
|Nov 19||13||INTERVIEWS; New tissue prep.
|Nov 26||14||Required tissues
|Dec 3||15||Required tissues
|Dec 10||16||Second set of micrographs
Please note that this is a tenative syllabus. Laboratory assignments
and notices will be posted on the top right side of the lab. blackboard.
It is the students responsibility to check the board for any changes to
the syllabus, announcements of new assignments and/or changes in dates that
various assignments are due
BIL 160 Evolution and Biodiversity, Spring 1997.
BIL 555 Projects in Electron Microscpy, Spring 1997.